Tissue Culture Laboratory

Badak LNG concerns on biodiversity of plants and animals which can be found on the forest, mangrove, and marine ecosystem. Some species living on these ecosystems are endangered plants like: Borneo ironwood (Eusideroxylon zwageri), Shorea sp., and several orchids like: Dendrobium, black orchid (Coelogyne pandurata), the spectacular giant orchid (Grammatophyllum speciosum), and Vanda. Cultivation of these species is needed for the stability and continuity of biodiversity in the company area. The most advanced method for cultivation is plant tissue culture.

 

 Through plant tissue culture, plant tissues (leaf, stem, root, seed) are grown on artificial media (agar) with in vitro environment. This technique can be used to grow new plants on artificial nutrient media through use of plant parts or cells. Plant culture must be conducted aseptically, otherwise the contamination could kill the entire plant. To keep the work place sterilized, UV germicidal lamps and alcohol sprayer are used.

 

The steps of plant culture are: sterilization of media and work area, preparation of media, initiation/inoculation in laminar airflow cabinet, incubation phase, subculture, rooting, and acclimatization process. All the equipments and work area must be sprayed with alcohol 70%. The media used in plant tissue culture are combination of agar, sugar, and nutrients (MS, VW, and WPM) with the addition of plant hormone, if necessary. All prepared agar media must undergo a sterilization process in autoclave. Initiation or inoculation is the next step, which plant tissues are put in agar media. This work must be conducted in sterile laminar airflow cabinet. If the plants grow well in the media, they can be divided and transferred into multiple new media (multiplicated) for further growth. This process is called subculture. All of the plants were stored in incubation room with adjusted light and humidity until the roots are formed. When the plants have grown complete organs, they can be transferred outside world through the acclimatization or adaptation process. This process is critical, because it will determined the fate and survival of plants.

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