Tissue Culture Laboratory

 

Plant tissue culture plants must be incubated in a sterile chamber after the initiation or inoculation process. In incubation room, the plants can grow well if the light, temperature, and humidity are properly adjusted. Some plant tissues grow well under low light condition (approx. 1000 lux). Light and dark periods are needed for regeneration. Regenerated plant need well lighted (approx. 3000 lux) condition with 16 hours light and 8 hour dark period. The illumination in the culture room is provided by cool white fluorescent light placed approximately 18″ above the culture racks. The temperature are set on the range between 20-25°C. The humidity are set on the range of 50-80%. The sterile condition of this room is important to prevent contamination, especially from dust or insect. Therefore, alcohol 70% must be sprayed regularly.

 

Badak LNG plant culture laboratory has succeeded in growing various types of plants. Of the many types that exist, the main focus of this lab is on rare and endemic plants from the island of Borneo.

 

INCUBATION ROOM (LABORATORY)

No

Year

Number of living explants in the incubation room

Number of types of explants native to Kalimantan

Explants native to Kalimantan

1

2017

34

2

Anggrek Hitam  (Coelogyne pandurata)

Kantong Semar (Nepenthes ampullaria)

2

2018

274

4

Anggrek Hitam (Coelogyne pandurata)

 Anggrek Tebu (Grammatophyllum speciosum)

Anggrek Tanah (Spathoglottis plicata)

Anggrek kelip (Phalaenopsis bellina)

3

2019

327

5

Anggrek Hitam (Coelogyne pandurata)

Anggrek Tebu (Grammatophyllum speciosum)

Anggrek Macodes (Macodes sp)

Anggrek Tanah (Spathoglottis plicata)

Anggrek kelip (Phalaenopsis bellina)