- PLANT OPERATION
- LNG CENTER OF EXCELLENCE
- MEDIA & EVENT
Tissue culture, which needs only a piece of the plant that faces extinction to build a large population, was deemed the best for conservation.
Badak LNG concerns on biodiversity of plants and animals which can be found on the forest, mangrove, and marine ecosystem. Some species living on these ecosystems are endangered plants like: Borneo ironwood (Eusideroxylon zwageri), Shorea sp., and several orchids like: Dendrobium, black orchid (Coelogyne pandurata), the spectacular giant orchid (Grammatophyllum speciosum), and Vanda. Cultivation of these species is needed for the stability and continuity of biodiversity in the company area. The most advanced method for cultivation is plant tissue culture.
Through plant tissue culture, plant tissues (leaf, stem, root, seed) are grown on artificial media (agar) with in vitro environment. This technique can be used to grow new plants on artificial nutrient media through use of plant parts or cells. Plant culture must be conducted aseptically, otherwise the contamination could kill the entire plant. To keep the work place sterilized, UV germicidal lamps and alcohol sprayer are used.
The steps of plant culture are: sterilization of media and work area, preparation of media, initiation/inoculation in laminar airflow cabinet, incubation phase, subculture, rooting, and acclimatization process. All the equipments and work area must be sprayed with alcohol 70%. The media used in plant tissue culture are combination of agar, sugar, and nutrients (MS, VW, and WPM) with the addition of plant hormone, if necessary. All prepared agar media must undergo a sterilization process in autoclave. Initiation or inoculation is the next step, which plant tissues are put in agar media. This work must be conducted in sterile laminar airflow cabinet. If the plants grow well in the media, they can be divided and transferred into multiple new media (multiplicated) for further growth. This process is called subculture. All of the plants were stored in incubation room with adjusted light and humidity until the roots are formed. When the plants have grown complete organs, they can be transferred outside world through the acclimatization or adaptation process. This process is critical, because it will determined the fate and survival of plants.Read More
Plant tissue culture plants must be incubated in a sterile chamber after the initiation or inoculation process. In incubation room, the plants can grow well if the light, temperature, and humidity are properly adjusted. Some plant tissues grow well under low light condition (approx. 1000 lux). Light and dark periods are needed for regeneration. Regenerated plant need well lighted (approx. 3000 lux) condition with 16 hours light and 8 hour dark period. The illumination in the culture room is provided by cool white fluorescent light placed approximately 18″ above the culture racks. The temperature are set on the range between 20-25°C. The humidity are set on the range of 50-80%. The sterile condition of this room is important to prevent contamination, especially from dust or insect. Therefore, alcohol 70% must be sprayed regularly.Read More
Acclimatization is one of the most important phase in plant tissue culture. After all plants have grown complete organs in the incubation room, they must transferred into the real world condition. Because, living in laboratory or in vitro environment, their organs developing a little deficiency, like thin cuticle in leaf that promote higher evaporation. Acclimatization process helps the plant to adapt more and developed stronger organs for higher chance of survival. This process are conducted in the green house under the filtered sun rays. The temperature and humidity are set by watering the green house regularly.Read More